LAI-1-dependent inhibition of cell migration requires IQGAP1 and Cdc42.
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(A) Confluent cell layers of A549 epithelial cells were treated with siRNA against IQGAP1, Cdc42, RhoA or Rac1 for 2 days. The cells were then treated or not with LAI-1 (10 μM, 1.5 h), scratched and let migrate for 24 h. Prior to imaging (0, 24 h), the detached cells were washed off. (B) The scratch area was quantified using ImageJ software at 7 different positions per condition in triplicate samples. Means and standard deviations of triplicate samples are shown (**p < 0.01). The data is representative of 3 independent experiments.
创建时间：
2016-02-22
