X-Ray induced DNA-Hydroxymethylation changes

In this study we addressed the question whether changes of DNA hydroxymethylation after X-ray irradiation occur in a random fashion or if they show preferences for specific regions or features. We did not only focus on gene loci and promotor sights but also on gene body hydroxymethylation alterations and associated changes in gene expression. A more profound insight into hydroxymethylation characteristics triggered by radiation could provide clues to mechanisms and consequences of cellular response to irradiation including the immediate consequence of DNA strand breaks associated with active DNA demethylation as well as the long term risk of tumorigenesis or other associated diseases. Overall design: Analysis of DNA-hydroxymethylation changes after X-ray irradiation of IMR90 cell line 1 to 120 h after exposure and evaluation of associated gene expression changes.