Screening results.

HDQ-P1 cells were exposed to the compounds in combination with 50 μM G418. After 72 h, cells were fluorescently labeled using anti-p53 antibody, and TP53 PTC readthrough was assessed using quantitative automated p53 immunofluorescence microscopy. The number of cells measured in 12 fields in each well (Valid Object Count) and p53 immunofluorescence (MEAN-CircAvgInten) are displayed. The numbers for Valid Object Count and p53 MEAN-CircAvgInten of untreated cells (mean ± SD; n > 100) were 2,272 ± 714 and 29.66 ± 4.36, respectively. The numbers for Valid Object Count and p53 Mean-CircAvgInten of G418-treated cells (mean ± SD; n > 100) were 2,284 ± 708 and 31.32 ± 4.12, respectively. Compounds with high toxicity (<2,000 cells) were removed from the list and the remaining compounds were sorted by p53 signal intensity.
(XLSX)