Single nucleus RNA-seq atlas of macaque ventromedial and dorsomedial hypothalamus

To uncover the cellular architecture of the macaque ventromedial (VMH) and dorsomedial hypothalamus (DMH), we used single nucleus RNA-seq (snRNA-seq) from a Rhesus macaque A 6.1 year-old Rhesus macaque had been maintained on chow prior to sacrifice and microdissection of the VMH and DMH. The VMH and DMH were then combined prior to nuclei isolation and snRNA-seq using the 10x Genomics 3’ gene expression platform.