Expression of genes following miR-19b-3p mimic treatment

In general, effect of treatment of cells with miRNAs is evaluated by down regulation of a target proteins, however miRNAs have hundreds of targets mRNA. Therefore, RNAseq of miRNA treated cells and control mimic treated cells provide good insight on how miRNA works in the cells. This data set is RNAseq following incubation of bovine granulosa cells with miR-19-3p. Granulosa cells were collected from antral follicles of ovaries and the granulosa cells were incubated in TCM-199 medium containing exosome free FCS and 60nM of mimic miRNAs (m miR-19b-3p or control mimic) for 2 days. Then RNA in the granulosa cells were extracted using RNAqueous (Invitrogen).RNA quality and concentration were examined using a Bioanalyzer (Agilent technologies, Palo Alto, CA, USA); a cDNA library was constructed using an NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) for RNA from FFs. The multiplied samples were sequenced as 75 bp reads (single read) on the NextSeq 500 (Illumina, San Diego, CA, USA). Image analysis, base calling and quality filtering were performed using the bcl2fastq2 v2.18.0.12 (Illumina) following the manufacturer's instructions. Sequence data were filtered to discard the adapter sequence, ambiguous nucleotides and low-quality sequences. The remaining sequence data were aligned to the Bos Taurus genome sequence (ARS-UCD1.2/bosTau9) to count sequence reads.