Fatty acid hydroxylation in transgenic Arabidopsis thaliana
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RcCb5-2 T1 events.csvHydroxy fatty acid (HFA) percent from T2 seed from T1 events of RcCb5-2 transformed into CL37. Data are presented in Figure 3A.
RcCb5-3 T1 events.csvHydroxy fatty acid (HFA) percent from T2 seed from T1 events of RcCb5-3 transformed into CL37. Data are presented in Figure 3B.
RcCb5-4 T1 events.csvHydroxy fatty acid (HFA) percent from T2 seed from T1 events of RcCb5-4 transformed into CL37. Data are presented in Figure 3C.
RcCBR1 RcCb5s T1 events.csvHydroxy fatty acid (HFA) percent from T2 seed from T1 events of RcCBR1+RcCb5 transformed into CL37. Data are presented in Figure 5.
AtCBR1 T1 events.csvHydroxy fatty acid (HFA) percent from T2 seed from T1 events of AtCBR1 transformed into CL37. Data are presented in Supplementary Figure 1.
RcCb5s T2 lines.csvHydroxy fatty acid (HFA) percent from T3 seed from T2 events of RcCb5s transformed into CL37. Data are presented in Figure 4 and Supplementary Table 1.Please note that a colon “:” is normally used in the x:y (number of carbon:number of double bonds) nomenclature of the fatty acids, however to avoid the use of the colon in this dataset we have the carbon number followed by the letter “c” and then number of double bonds. For example “18c 1” (18:1) is oleic acid.
RcCBR1 RcCb5 selected lines.csvHydroxy fatty acid (HFA) percent from T3 seed from T2 events of RcCBR1+RcCb5s transformed into CL37. Data are presented in Figure 6.
Q-PCR data.csvQuantitative reverse-transcriptase-PCR of developing castor bean endosperm. Transcripts were normalized to RcACTIN. Error bars represent standard error of the mean of three independent experiments with three replicates each. Relative fold change = 1/(2^(experimental-control). Data are presented in Figure 2.
RcCb5-2 T1事件.csv:RcCb5-2经CL37转化后T1事件中T2种子的羟基脂肪酸(HFA)百分比。数据展示于图3A。
RcCb5-3 T1事件.csv:RcCb5-3经CL37转化后T1事件中T2种子的羟基脂肪酸(HFA)百分比。数据展示于图3B。
RcCb5-4 T1事件.csv:RcCb5-4经CL37转化后T1事件中T2种子的羟基脂肪酸(HFA)百分比。数据展示于图3C。
RcCBR1 RcCb5s T1事件.csv:RcCBR1与RcCb5转化后T1事件中T2种子的羟基脂肪酸(HFA)百分比。数据展示于图5。
AtCBR1 T1事件.csv:AtCBR1转化后T1事件中T2种子的羟基脂肪酸(HFA)百分比。数据展示于补充图1。
RcCb5s T2线.csv:RcCb5s转化后T2事件中T3种子的羟基脂肪酸(HFA)百分比。数据展示于图4及补充表1。请注意,在脂肪酸的x:y(碳数:双键数)命名法中,通常使用冒号“:”,然而为了避免在此数据集中使用冒号,我们采用碳数后跟字母“c”再接双键数。例如,“18c 1”(18:1)代表油酸。
RcCBR1 RcCb5选定的线.csv:RcCBR1与RcCb5s转化后T2事件中T3种子的羟基脂肪酸(HFA)百分比。数据展示于图6。
Q-PCR数据.csv:发展中的蓖麻籽内胚乳的定量逆转录PCR。转录物以RcACTIN为标准进行归一化。误差线表示三次独立实验中每次三个重复的标准误差。相对变化倍数=1/(2^(实验值-对照值))。数据展示于图2。
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