Induction of CTH expression in response to amino acid starvation confers resistance to anti-LAT1 therapy in MDA-MB-231 cells

We examined gene expression change under amino acid restriction in JPH203 treated MDA-MB-231 and T-47D cells. JPH203 treatment induced endoplasmic reticulum stress response via amino acid starvation stress pathway in both MDA-MB-231 and T-47D cells. Moreover, MDA-MB-231 was upregulated anti-oxidants related genes. To determine JPH203 treatment regulated genes, total 4 samples were derived from MDA-MB-231 or T-47D cells stimulated with 100μM of JPH203 for 24 hours. 100μM of Captisol was used as control.