RNA-affinity isolations 13 RBPs

To identify RNAs specifically associated with potential RBPs, yeast cells expressing TAP-tagged RBP or the wild-type strain BY4741 (mock control) were grown to mid-log phase in rich medium and harvested by centrifugation为鉴定与潜在RNA结合蛋白（RNA-binding proteins, RBPs）特异性结合的RNA，将表达TAP标签标记的RBP的酵母细胞以及野生型菌株BY4741（空白对照）接种至丰富培养基中培养至对数中期，随后通过离心收集细胞。