Detection and monitoring of insect in bioaerosols

Studies on bioaerosol have primary focused on their chemical and biological compositions, impact on public health and ecosystem. However, most studies have only focus on viruses, bacteria fungi and pollen. To assess the diversity and composition of airborne insect material in particulate matters (PMs), we attempted to detect DNA traces of insect origin in dust samples collected over a two-year period. These samples were systematically collected at one-month intervals and categorized into two groups, PM2.5 and PM10, based on the aerodynamic diameter of the aerosol particles. Cytochrome-c oxidase I was the barcoding region used to identify the origins of the extracted DNA