Comprehensive Characterization of Protein Turnover by Comparative SILAC Labeling Analysis in 3T3-L1

A balance
between the synthesis and degradation of proteins is
referred to as protein turnover, which is crucial for cellular protein
homeostasis. Proteome-wide analysis of protein turnover in adipocytes,
which are well-known for their role in energy storage and their link
to obesity and metabolism disorders, is yet to be conducted. Thus,
with this objective in mind, our investigation utilized a comparative
analysis of time-dependent SILAC labeling to assess protein turnover
in 3T3-L1 adipocytes, spanning a period of 0 to 144 h. We observed
that relatively faster or slower protein half-lives in several protein
groups were associated with the PPARγ signaling pathway, energy
metabolism, extracellular matrix, ubiquitin–proteasome system,
RNA splicing, Golgi complex, and lysosome. It is anticipated that
these protein half-life profiles will provide greater clarity on the
life cycle of adipocyte proteome and shed light on how they maintain
protein homeostasis.