Comparison of NK cells and ILC1 in lean versus obese mouse livers by RNASeq. Comparison of NK cells and ILC1 in lean versus obese mouse livers by RNASeq

Mice were weaned onto standard RM1 diet or onto a highly palatable obesogenic diet (824018 – ‘45% AFE fat) supplemented with condensed milk. After 12 weeks, mice were killed, and liver NK cells (Lin- NK1.1+ CD49a- CD49b+) or ILC1 (Lin- NK1.1+ CD49a+ CD49b-) were sorted. Total RNA was extracted from sorted cells, cDNA generated and RNASeq performed. Overall design: Cells from the livers or 3 lean and 3 obese mice were sorted, with liver NK cells and ILC1 isolated from each mouse, for a total of 12 samples