RNA sequencing in hepatocytes and DR-derived hepatocytes

During chronic liver injury, the ductular reaction generates hepatocytes. The aim of the analysis is to compare the phenotype of the DR-derived hepatocytes with that of native hepaotcytes in the same injured liver and control healthy hepatocytes. Overall design: We compared 5 biological replicates of each of the 3 populations. Due to technical constraints 10 samples were processed in a first experiment (Experiment 1) and 9 samples were processed in a second experiment (experiment 2). Log fold-change obtained from both experiment were highly correlated (r ~ 0.95). Several of the same samples were processed in both Experiment 1 and Experiment 2: CTL 5, RED 2, RED 3, RED 4. For these samples, independent libraries were made for each Experiment following methods outlined in the Extraction protocol on the sample pages.