Transcriptomic analysis of feline chronic gingivostomatitis (FCGS)

The purpose of this study was to characterize the transcriptomic alterations accompanying the inflammation involved in feline chronic gingivostomatitis (FCGS). Towards this goal next-generation sequencing (NGS)-based gene expression profiling (RNA-Sequencing; RNA-Seq) was performed on matched pairs of FCGS diseased and healthy tissues obtained from three feline subjects. A total of 6 total RNA samples were analyzed in this study. The study included matched pairs of inflamed feline chronic gingivostomatitis (FCGS; diseased) and adjacent healthy mucosal tissues from the area lateral to the palatoglossal folds from 3 individual feline subjects: Bart, Jerome, and Sadie. Full-thickness 6-8 mm biopsies were obtained from inflamed and adjacent healthy oral mucosa and were collected under general anesthesia. Tissues were snap-frozen in liquid nitrogen, total RNA was isolated, and then samples submitted for RNA-sequencing analysis.