High depth sequencing of BrU-labeled nascent RNA in METTL3 depleted cells.
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下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP148114
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资源简介：
BrU incorporation into nascent RNA followed by immunoprecipitation with a BrU specific antibody. The nascent RNA was subsequently eluted with BrU competition. Overall design: HEK293T cells, treated with METTL3 siRNA, were incubated with 2 mM bromouridine for 15 min labeling time and chased with 20 mM uridine for 60 min. Nascent RNA was purified during BrU-IP with anti-BrdU.
创建时间：
2022-10-20
