Primary fibroblasts were extracted from the ears/pinnae of three young C57BL/6 mice of each sex (male and female) from the NIA. Cells were frozen at the end of passage 3 and then thawed for analysis b
Sample multiplexing in flow cytometry is a powerful technique which allows for reduction of error, inclusion of control samples for batch effect correction, and reduction in both time and consumable u
Intracellular ROS formation was measured using flow cytometry in yeasts loaded with H2DCFDA. Samples were treated with APCP for 2 minutes and analyzed at 30, 60 and 120 seconds after treatment. Data a
