Dinoflagellate-associated bacteria metagenomics. uncultured bacterium

Coolia monotis and Ostreopsis ovata are armoured, marine, benthic dinoflagellate species. In tropical and subtropical seas, these dinoflagellates were among the genera found in ciguatera affected areas. Generally, ciguatera fish poisoning is associated with tropical reef fish, especially demersal herbivorous and carnivorous fish of coral reefs and pelagic carnivores, such as mackerels and barracudas. When organisms are rapidly exposed to high concentrations of these toxic blooms, shellfish-poisoning events, significant public health problems, and mass mortalities of aquatic organisms can result. This marine algal toxicity is very much related to the associated bacterial assemblages. These samples were directly extracted from the clonal culture of these dinoflagellates. With the long-term aim of understanding the role of bacterial flora has in bloom development and toxicity of these benthic dinoflagellates, we access the phylogenetic diversity of associated bacterial assemblages using genomic-based approach. In addition, a fosmid library of the same environmental samples had been constructed to explore the distribution and diversity of metabolic pathways in these communities. FJ644583-FJ644618 correspond to 16S ribosomal RNA sequences isolated from this study.