Silencing of lnc-klhdc7b in the HCC1187 cell line. Silencing of lnc-klhdc7b in the HCC1187 cell line

By using shRNAs the lnc-klhdc7b was silenced in the cell line HCC1187, the shRNA-1 carried a ~75% silencing compared to the negative control (NC). We evaluated the role of LncKLHDC7B on oncogenic phenotypes in the IM breast cancer HCC1187 cell line model. The knockdown of LncKLHDC7B was achieved with ~75% of silencing efficiency. A complete genomic analysis of the transcriptomic state after silencing of LncKLHDC7B revealed an impact on the global expression of transcripts Overall design: We analyzed expression profile from HCC1187 cell line silenced by shRNA-1 versus lnc-KLHDC7B using the Affymetrix Human Transcriptome Array 2.0 platform. Array data was processed by Affymetrix Exon Array Computational Tool.