Comparison of gene profile of WT, STAT3 KO and STAT3koPD-1ko donor T cells in the liver of allogenetic HCT mouse recepients

We injected Thy1.2+ T cells from WT or STAT3ko or STAT3koPD-1ko C57BL/6 mice with T cell-depleted bone marrow cells from WT C57BL/6 mice into WT BALB/c recipients to establish acute GVHD model. Overall design: We isolated donor CD4+ or CD8+ T cells from liver on day 6 after HCT, then performed RNA-Seq to compare gene profiling of T cells from WT, STAT3 KO or STAT3koPD-1ko donors.