Figure 1 Phylogeny of Dacrymycetaceae and related species generated by MP analysis based on the combined ITS and nrLSU dataset.

For the phylogenetic reconstruction of Dacrymycetaceae, the combined ITS + nLSU dataset included 174 fungal specimens representing 113 species. In the phylogeny, a total of 69 new sequences of Dacrymycetaceae were generated, including 38 from the ITS region and 31 from the nrLSU region. The aligned dataset contained 3485 characters (including gaps), consisting of 2301 constant sites, 273 variable and parsimony-uninformative sites, and 911 parsimony-informative sites. MP analysis yielded 5,000 parsimonious trees (TL = 2,354, CI = 0.555, RI = 0.822, RC = 0.456, HI = 0.445). Bayesian inference (BI) analyses were run for 40 million generations, with an average standard deviation of split frequencies = 0.009021, and both maximum likelihood (ML) and Bayesian inference (BI) analyses produced topologies congruent with the maximum parsimony (MP) tree. Only the MP tree is shown with bootstrap values for BP, BS and BPP indicated at corresponding nodes (Fig. 1). Bootstrap support values for MP and ML greater than 50% and BI greater than 0.95 are given at each node. The newly generated sequences are in bold.