Spatial Multimodal Analysis: MALDI-MSI and Spatial Transcriptomics within the same tissue section

We present a spatial omics approach that merges and expands the capabilities of independently performedin situassays on a single tissue section. Our spatial multimodal analysis combines histology, mass spectrometry imaging, and spatial transcriptomics to facilitate precise measurements of mRNA transcripts and low-molecular weight metabolites across tissue regions. We demonstrate the potential of our method using murine and human brain samples in the context of dopamine and Parkinson’s disease. Tissue sections processed with MALDI-MSI were eventually analyzed using Visium. Controls for some of the samples are included. *** From Submitter: We are restricted to deposit raw sequencing from humans to SRA. Those will be deposited on the Swedish federated EGA node and we will link when it became available to us. ***