Measuring the effect of pan-Rho inhibition on microtubule destabilization response

In this study, we sought to measure what changes cells experienced upon microtubule destabilization after inhibiting Rho GTPases. We used human pulmonary microvascular endothelial cells (HPMECs) as a model to measure cell response to microtubule depolymerization in a sensitive cell type. RNA-seq profiling of primary human pulmonary microvascular endothelial cells (HPMECs). First, cells were pre-treated with 1 µg/ml cell-permeable C3 transferase (Rho inhibitor, hereafter labeled RHOi) for 4 hours. Following pre-treatment, cells were treated with either 0.1% DMSO or 100 nM vinblastine for 6 hours, while concurrently adding an additional 0.5 µg/ml C3 transferase (RHOi) booster dose. Cells from three donors were purchased from PromoCell. Donor identification between the metadata and data file names corresponds as follows: donor 1 = donor 1, donor 2 = donor 4, donor 3 = donor 6.