Fractionation iCLIP detects persistent SR protein binding to conserved, retained introns in chromatin, nucleoplasm and cytoplasm. Mus musculus
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA317002
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资源简介:
We report a new method which combines subcellular fractionation to advance sequencing technique iCLIP. In this way we identified the spectrum of interactions of two SR-proteins (SRSF3 and SRSF7) to their target RNAs in different subcellular compartments Overall design: Examination of 4 replicates for Cytoplasm, Nucleoplasm and Chromatin fractions using Fr-iCLIP technique for SRSF3-GFP; examination of 3 replicates for Cytoplasm, Nucleoplasm and Chromatin fractions using Fr-iCLIP for SRSF7-GFP
创建时间:
2016-03-31



