Effects of <italic>Morinda officinalis</italic> How. on Gut Microbiota in Mice with Chronic Restraint Stress Depression

Objective: To investigate the effects and mechanisms of Morinda officinalis How. on the gut microbiota of chronic restraint stress (CRS) depression model mice. Methods: Eight-week-old male Kunming mice were randomly divided into six groups based on their body weight: control, model, fluoxetine, and M. officinalis How. low- (25 mg/kg/d), medium- (50 mg/kg/d), and high-dose (100 mg/kg/d) groups were established. CRS was used to establish a mouse model of depression. Depression-like behavior was observed using the sucrose preference (SPT) and tail suspension tests (TST). Morphological changes in the liver, kidneys, and testes were observed using hematoxylin and eosin (HE) staining. Serum 5-hydroxytryptamine (5-HT) levels were measured using enzyme-linked immunosorbent assay (ELISA). Finally, the gut microbiota were analyzed using 16S rRNA gene amplicon sequencing. Results: Compared with the control group, the sucrose preference rate of mice in the model group was significantly reduced, tail suspension time was significantly prolonged (PPM. officinalis How. showed good antidepressant effects. Its sucrose preference rate was significantly increased, and tail suspension time was significantly shortened (PPPOscillibacter, Eubacterium_xylanophilum_group, Kurthia and unidentified bacterium were increased (PM. officinalis How. regulates gut microbiota imbalance and improves depression-like behavior in CRS mice.