A multi-omics approach to elucidate okadaic acid-induced changes in human HepaRG hepatocarcinoma cells

Okadaic acid (OA) is a marine biotoxin that can induce diarrheic shellfish poisoning upon consumption. It can accumulate in filter-feeding shellfish, thereby reaching the human food chain. In addition to inducing severe gastrointestinal symptoms, OA can pass the intestinal barrier into the bloodstream, thereby reaching the liver. However, the effects of OA on the liver have not been much in the focus of research so far. Therefore, this study now focuses on elucidating changes in the proteome of HepaRG cells induced by OA. Furthermore, as a potent phosphatase inhibitor, we expected changes in the phosphoproteome of the cells. Therefore, we further investigated the phosphoproteome of the HepaRG cells after OA exposure. Results revealed changes in enzyme activity regulation, cytoskeleton organization and energy metabolism.