LeafContourEFD: a reproducible workflow for biologically oriented elliptic Fourier analysis of leaf shape

This Dryad dataset contains image- and outline-based leaf morphometric data for Quercus serrata, Quercus crispula, and Triadica sebifera, generated to support reproducible elliptic Fourier analysis using the LeafContourEFD workflow. The dataset includes scanned leaf images, region-of-interest (ROI) images indicating the sampled leaves on the original scans, cropped ROI images, binarized leaf mask images before and after manual correction, contour coordinate CSV files, metadata files in CSV and JSON formats, Fourier coefficient CSV files, and oriented true EFD normalized CSV files. For the Quercus dataset, an additional species correspondence table link image file names with species names.
, Leaf images of Quercus serrata, Quercus crispula, and Triadica sebifera were scanned and processed using LeafContourEFD , a napari-based GUI application for outline-based morphometric analysis. For each scan, region of interest (ROIs) corresponding to individual leaves were defined, cropped, and converted to binary mask images. Binary mask were manually corrected when necessary, and leaf contour coordinates were then extracted from the final masks. Elliptic Fourier descriptors (EFDs) and oriented true EFD normalized coefficients were calculated from the extracted contours. Metadata describing processing steps and output files were exported in CSV and JSON formats. Subsequent data processing and analysis were conducted using scripts archived in a separate repository (GitHub repository).
, # Data from: LeafContourEFD: a reproducible workflow for biologically oriented elliptic Fourier analysis of leaf shape

Dataset DOI: [10.5061/dryad.6t1g1jxdh](https://doi.org/10.5061/dryad.6t1g1jxdh)

## Description of the data and file structure

These data were collected as part of a study on reproducible leaf shape quantification using elliptic Fourier analysis. They were generated to document the processing workflow implemented in [LeafContourEFD](https://github.com/maple60/leaf-contour-efd), to compare normalization procedures, and to support downstream multivariate analyses of leaf outlines from *Quercus serrata*, *Q. crispula*, and *Triadica sebifera*.

Folder names indicate dataset type and taxonomic group: folder ending in `quercus` contain data for *Q. serrata* and *Q. crispula*, whereas folders ending in `triadica_sebifera` contain data for *T. sebifera*. These files document the major intermediate and final outputs produced by LeafContourEFD, from image selection and segment..., ,