New methods of structure refinement for macromolecular structure determination by NMR
收藏PubMed Central1998-05-26 更新2026-05-02 收录
下载链接：
https://pmc.ncbi.nlm.nih.gov/articles/PMC34492/
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资源简介：
Recent advances in multidimensional NMR methodology have permitted solution structures of proteins in excess of 250 residues to be solved. In this paper, we discuss several methods of structure refinement that promise to increase the accuracy of macromolecular structures determined by NMR. These methods include the use of a conformational database potential and direct refinement against three-bond coupling constants, secondary (13)C shifts, (1)H shifts, T(1)/T(2) ratios, and residual dipolar couplings. The latter two measurements provide long range restraints that are not accessible by other solution NMR parameters.
提供机构：
National Academy of Sciences
创建时间：
1998-05-26
