Correlations of m6A Methylation-Related mRNAs with Thyroid Cancer

The specific role of m6A modification in the malignant progression of papillary thyroid carcinoma (PTC) without autoimmune thyroid disease (AITD) remains unclear. We collected cancerous and para-cancerous tissues and associated clinical information form PTC patients without AITD to both identify differentially expressed genes and quantify m6A levels in the mRNA and ncRNA transcripts. We generated the following comparisons using Fold-change (FC) ≥ 1.5 or ≤ 0.5, and p < 0.05 were used to identify the differentially m6A methylated RNAs.The total RNAs were immunoprecipitated with anti-N6-methyadenosine (m6A) antibody. The modified RNAs were eluted from the immunoprecipitated magnetic beads as the “IP”. The unmodified RNAs were recovered from the supernatant as “Sup”. Two channel arrays were used for these samples but the data were analyzed as single-channel so that each sample represents either IP or supernatent and the sample table includes the normalized signal values.