Immune repertoire sequencing in AML blasts

Immunoglobulin (Ig) is traditionally known as a hallmark of B lymphocytes exerting antibody functions. However, our previous studies demonstrated that acute myeloid leukemia (AML) cells could also express Igs with distinct roles. Since the function of the Ig is highly correlated with its V region characteristics, we performed a comprehensive sequencing analysis of AML-derived Ig repertoire in 16 patients. Ig heavy chains and light chains were amplified for each sample by combined multiplex PCR and next-generation sequencing (NGS) (Illumina Miseq, 2 x 250bp).