H3K27me3 MFI (Fig. 4G & Fig S6N)
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https://figshare.com/articles/dataset/H3K27me3_MFI_Fig_4G_Fig_S6N_/29918531
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资源简介：
Juveniles were dissociated in pools of 15 polyps, using Trypsin/formaldehyde. Cells were stained with 1μg/ml FxCycle violet DNA dye (Invitrogen), mOr2 was detected with anti-dsRed (Mouse, Takara Bio Clontech 632543) and goat-anti-mouse Alexa568, H3K27ac and H3K27me3 were detected with H3K27ac (Rabbit, Abcam, Ab4729) and H3K27me3 (Rabbit, Diagenode, C15410068) and and goat-anti-rabbit Alexa488.
Flow cytometry was performed a BD LSRFortessa (BD Life Sciences) and the mOr2 and chromatin mark labelled cells in samples were determined based on Alexa568 and Alexa488 fluorescence in comparison with the negative controls on cells within the cell cycle (based on DNA staining) using FlowJoV10.8 (BD Life Sciences).
.wsp files contain the samples, gating strategy and cell populations used for the analysis in FlowJo
.fcs files represent the individual flow cytometry output files that were analysed in the .wsp file
创建时间：
2025-08-15
