Comparison of gene expression profile in E. coli BW25113 wild type and ldhA-pflB- double mutant growing microaerobically in M9 media containing 10 g/L Glucose Overall design: two samples: wild type an
Golden Gate cloning is a prominent DNA assembly tool in synthetic biology for the assembly of plasmid constructs often used in combinatorial pathway optimization, with a number of assembly kits develo
D-lactic acid is a three-carbon organic acid with a chiral structure and can improve the thermostability of polylactic acid. Microorganisms such as the methylotrophic yeast Pichia pastoris, which lack
To solve the problem of low FK520 production by Streptomyces hygroscopicus var. ascomyceticus FS35, PHB synthesis gene phaC and PHB decomposition gene fkbU were co-overexpressed in parent strain FS35