single-cell RNA sequencing of in vivo matured and maturation deficient human oocytes

Single cell transcriptomic composition of in vivo matured (IVO) MII oocytes and maturation deficient (MD) oocytes discloses the aberrant expression level of spliceosomal proteins, accompanying with differential alternative splicing (DAS) events. Using single-cell transcriptomic RNA sequencing (RNA-seq), differentially expressed genes (DEGs) between IVO and MD oocytes and enriched pathways were analyzed based on the polyadenylated transcripts abundance. Three MD oocytes and three normal IVO oocytes were derived from six individuals.