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Identification of proteins crosslinked to lysosomal sphingosine and cholesterol probes

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NIAID Data Ecosystem2026-03-14 收录
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https://www.omicsdi.org/dataset/pride/PXD039780
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资源简介:
HeLa cells were labelled with lysosomally localized photocrosslinkable and clickable probes for sphingosine and cholesterol. Upon photocrosslinking and cell lysis, the protein-lipid complexes were clicked to biotin azide and enriched via Streptavidin-immunoprecipitation. Enriched protein-lipid complexes were then analyzed by LC-MS/MS to identify proteins interacting with sphingosine and cholesterol, respectively.
创建时间:
2023-02-07
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