Identification of proteins crosslinked to lysosomal sphingosine and cholesterol probes
收藏NIAID Data Ecosystem2026-03-14 收录
官方服务：
资源简介：
HeLa cells were labelled with lysosomally localized photocrosslinkable and clickable probes for sphingosine and cholesterol. Upon photocrosslinking and cell lysis, the protein-lipid complexes were clicked to biotin azide and enriched via Streptavidin-immunoprecipitation. Enriched protein-lipid complexes were then analyzed by LC-MS/MS to identify proteins interacting with sphingosine and cholesterol, respectively.
创建时间：
2023-02-07
