Expression data from cultured spermatogonia established from 10 day old mouse testes

Spermatogonial stem cells (SSCs) undergo self-renewal division to sustain spermatogenesis. Although it is possible to derive germline stem (GS) cell cultures from most of the mouse strains by supplementing GDNF and FGF2, SSCs from a 129 background do not proliferate under the same culture conditions, which suggested that they have distinct self-renewal requirements. We modified previous culture conditions and established long-term culture of SSCs of 129 mice. 129 GS cells reinitiated spermatogenesis and produced offspring following transplantation into the seminiferous tubules of infertile mouse recipients. This dataset show the differences of gene expressions of GS cells between C57BL/6 and 129 mice, which have important implications in understanding requirements of self-renewal mechanisms. In this dataset, we include the expression data obtained from cultured spermatogonia (GS cells) derived from C57BL/6, and 129 mice. Each group contains 2 biological replicates. Overall design: GS cells were established from testes of 10 day old mice of C57BL/6 and 129 strains. Total RNA samples were extracted with TRIzol reagent (Invitrogen) and purified using the RNeasy cleanup system (QIAGEN, Valencia, CA). cDNA libraries were generated using a TruSeq stranded mRNA library preparation kit (Illumina, San Diego, CA). Sequencing was performed using NextSeq550 (Illumina) with a single-read sequencing length of 76 bp. 4 Total samples were analyzed.