Environmental influences on Streptococcus sanguinis membrane vesicle biogenesis

To look for transcriptomic changes that resulted in membrane vesicle biogenesis, we performed RNA sequencing on S.sanguinis in different culture and community conditions We then examined three separate differential expression analyses, as well as correlated the phenotypic vesicle quantification with normalized count data across all samples. Overall design: Comparative gene expression profiling analysis of RNA-seq data for S.sanguinis at late log phase growth under glucose and sucrose media, or with the presence of oral microbes C.durum, F.alocis, and P.gingivalis