five

Whole heart response to stable HIF-1alpha overexpression

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12673
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An oxygen stable form of HIF-1alpha was overexpressed in the hearts of mice, under tet-off control. Bi-allelic transgenic mice were created by crossing alpha-MHC promoter/tet transactivating protein expressing mice with tet responsive element promter/stable HIF-1alpha protein expressing mice. Mice were either, maintained on Doxycycline (inhibiting the expression of the HIF-1alpha transgene) or removed from doxycycline (inducing expression) for one or three days.
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2019-02-19
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