Acute pulmonary and splenic response in an <i>in vivo</i> model of whole-body low-dose X-radiation exposure

<b>Purpose:</b> Diagnostic radiation is an important part of patient care in the Intensive Care Unit; however, there is little data on the acute effects of exposure to these doses. We investigated pulmonary and splenic response 30 minutes, 4 hours or 24 hours after exposure to 2 mGy, 20 mGy, 200 mGy or 4 Gy whole-body X-radiation in a Sprague Dawley rat model. <b>Materials and methods:</b> Lung injury was assessed via respiratory mechanics, pulmonary edema, cellular, and proteinaceous fluid infiltrate and protein expression of oxidative stress markers. The radiation effect on the spleen was determined via proliferation, apoptosis and protein expression of oxidative stress markers. <b>Results:</b> All measurements of the lung did not differ from sham animals except for an increase in catalase after high dose exposure. Stimulated splenocyte proliferation increased after sham and low dose exposure, did not change after 200 mGy exposure and was significantly lower after 4 Gy exposure. The number of apoptotic cells increased 4 hours after 4 Gy exposure. There were fewer apoptotic cells after low dose exposure compared to sham. Both catalase and MnSOD were increased after 4 Gy exposure. <b>Conclusion:</b> There was no measured effect on pulmonary function while there was an impact to the spleen after low and high dose exposure.

**研究目的**：诊断辐射是重症监护病房（Intensive Care Unit, ICU）患者诊疗的重要组成部分，但目前针对此类辐射剂量暴露的急性效应相关研究数据仍较为匮乏。本研究以斯普拉格-道利大鼠（Sprague Dawley rat）为模型，探究暴露于2 mGy、20 mGy、200 mGy及4 Gy全身X射线辐射后30分钟、4小时及24小时时，肺部与脾脏的生物学应答变化。
**材料与方法**：通过呼吸力学检测、肺水肿评估、细胞及蛋白样液体浸润情况，以及氧化应激标志物的蛋白表达水平，对肺损伤程度进行评价；通过脾淋巴细胞增殖活性、凋亡情况及氧化应激标志物蛋白表达水平，分析辐射对脾脏的影响。
**研究结果**：除高剂量辐射暴露后过氧化氢酶（catalase）水平升高外，肺部各项检测指标与假照射组动物均无显著差异。假照射及低剂量辐射暴露后，受刺激的脾淋巴细胞增殖活性升高；200 mGy剂量暴露后增殖活性无明显变化；4 Gy剂量暴露后增殖活性则显著降低。4 Gy剂量暴露后4小时，凋亡细胞数量显著增加；低剂量辐射暴露组的凋亡细胞数量较假照射组更少。4 Gy剂量暴露后，过氧化氢酶与锰超氧化物歧化酶（MnSOD）的表达水平均升高。
**研究结论**：本研究未检测到辐射暴露对肺功能产生显著影响，但低剂量与高剂量辐射暴露均会对脾脏造成显著影响。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于低剂量全身X射线暴露的急性生物效应研究，使用Sprague Dawley大鼠模型，探究肺和脾在暴露后30分钟至24小时内的响应。研究发现，肺功能未受显著影响，而脾在低剂量和高剂量暴露下均表现出变化，包括增殖和凋亡的差异。数据集包含原始实验数据，支持开放获取，适用于辐射生物学和医学研究领域。