File S1 - Preparation of 5′-O-(1-Thiotriphosphate)-Modified Oligonucleotides Using Polymerase-Endonuclease Amplification Reaction (PEAR)

Figure S1, PAGE electrophoresis of PspGI digestions of PEAR products. (A) Digestion of natural PEAR products;(B) Digestion of PEAR products incorporating PS-dATP; (C) Digestion of PEAR products incorporating PS-dGTP. Figure S2, The nucleotide sequence of the recognition site of PspGI. Figure S3, Sanger sequencing of PEAR products. (Target sequence repeats are underlined in blue; PspGI restriction enzyme recognition sites are underlined in yellow.) Figure S4, The LC/UV Chromatogram and Deconvoluted Mass Spectrum of the *A*G PEAR product incorporating dATPαS+ dGTPαS fully digested by PspGI. Components: (A) RT = 7.45 min: MW = 6742.0; (B) RT = 8.00 min; (C) RT = 8.38 min; (D) RT = 8.76 min; (E) RT = 9.05 min; See Table S2 for detailed characterization of components. Table S1, Characterization components of the *A PEAR product by LC/UV/MS analysis. Table S2, Characterization components of the *A*G PEAR product by LC/UV/MS analysis.
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