Quantitative proteomics analysis of proteins with decreased abundance following glucose starvation.

Total lysates of control and glucose-starved E.histolytica trophozoites were produced and used as substrates for stable isotope dimethyl labeling of peptides for quantitative proteomics analysis. The list describes the proteins that were up-regulated (Table 2) or down-regulated (Table 3) following glucose starvation. Mean H/L provided directly from the MS analysis is the ratio between the protein levels in lysates from glucose-starved and control trophozoites. The value displayed for pepCount represents the number of peptides used for protein identification and statistical value is given in the column Ppro (protein probability). Ppro is the probability of the best peptide match (the peptide with the lowest score) for each identified protein. Classification and characterization of the domains in the hypothetical proteins was done using motif Search (http://motif.genome.jp/) of the PROSITE and Pfam databases.