J-line Chickens Brain Isoseq
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下载链接:
https://www.ncbi.nlm.nih.gov/sra/ERP014795
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资源简介:
Brain tissue from J-line chickens was collected and RNA was extracted. The RNA was selected for poly-A tails. Normalization by denature/renature was applied to synthesized cDNA. The cDNA were then size selected for 1kb and 2kb lengths using Ampure beads. For the 01kb cDNA, 11 SMRT cells were used. For the 2kb cDNA, 14 SMRT cells were used. Sequencing was performed on an RSII Pacific Biosciences machine using P4C2 chemistry.
创建时间:
2018-02-21



