Gene expression profiling of cancer cells purified from prostate cancer bone metastasis mouse model. Gene expression profiling of cancer cells purified from prostate cancer bone metastasis mouse model

The study aimed to determine mechanisms of macrophage promotion of anti-androgen resistance of prostate cancer bone disease. For this purpose, we developed a novel prostate cancer bone metastasis mouse model, MycCaP-Bo, via multiple rounds of in vivo selection of bone homing cells following intra-cardiac inoculation of a murine prostate cancer cell line, MycCaP cells. Cancer cells were purified from bone metastasis using fluoresent accelerated-cell sorting (FACS) based on their iRFP expression. Cancer cells were purified from treatment- naïve tumours, resistant tumours and resistant tumours with short-term depletion of macrophages. RNA was extracted from purified cancer cells and profiled using next-generation sequencing. Overall design: Gene expression profiles of cancer cells purified from different stages of prostate cancer bone metastasis mouse model; naïve tumours (vehicle-treated), naïve tumours (vehicle-treated plus macrophage depleted by liposome clodronate treatment for four days), resistant tumours (18-day Enzalutamide treated), and resistant tumours with short-term depletion of macrophages (18-day Enzalutamide treated plus macrophage depleted by liposome clodronate treatment in the last four days).