DNA methylation reprogramming after spinal nerve injury [RRBS]. DNA methylation reprogramming after spinal nerve injury [RRBS]

Purpose: Nerve injury-induced hyperactivity of primary sensory neurons in the dorsal root ganglion (DRG) contributes critically to chronic pain development, but its underlying mechanisms remain incompletely understood. Chronic neuropathic pain has a clear epigenetic component, however, most studies so far have focused on histone modifications. We determined changes of DNA methylation in the rat DRG, spinal cord, and prefrontal cortex after spinal nerve ligation (SNL). Overall design: We used a rat model of neuropathic pain induced by spinal nerve ligation (SNL). We analyzed DNA methylation in dorsal root ganglia (DRG) by the RRBS method (Reduced Representation Bisulfite Sequencing).