Human cytomegalovirus dysregulates neurodevelopmental pathways in cerebral organoids

This study investigated the viral mechanisms of fetal neural malformation using in vitro human cerebral organoids to model congenital CMV infection Overall design: Cerebral organoids cultured for 55 days followed by 14 days infection with CMV Merlin were harvested for bulk RNA-sequencing. Four biological replicates of CMV infected cerebral organoids were compared to 4 biological replicates of non-infected cerebral organdies.