Differential gene expression profiles of human promyelocytic leukemia cell lines exposed to benzene and its metabolites

The gene expression profiles of HL-60 cells were determined with IC50 doses of 3 chemicals (benzene, hydroquinone and benzoquinone) at a 3 h time point. Assays were performed in triplicate for each chemical/dose, and individual gene-expression profiles were determined for each chemical at the tested concentration doses. SUBMITTER_CITATION: http://www.sciencedirect.com/science/article/pii/S1382668911001049, DOI: doi:10.1016/j.etap.2011.06.001, Reference: ENVTOX 1446 Three independent experiments were performed for each chemical (N = 6), simultaneously. Labeling and hybridization were performed using the instructions of the Platinum Biochip Reagent Kit (GenoCheck Co. Ltd., Korea). This was followed by the coupling of the Cy3 dye for the controls (DMSO) and Cy5 dye for the treated samples. Hybridization was performed in a hybridization oven at 62 oC for 12 h. After washing (2× SSC/0.1% SDS for 2 min at 58 oC, 1× SSC for 2 min at RT and 0.2× SSC for 3 min at RT), the slide was dried by centrifugation at 800 rpm for 3 min at RT. Hybridization images on the slides were scanned by ScanArray Lite (PerkinElmer Life Sciences, USA). Scanned images were analyzed with GenePix 3.0 software (Axon Instruments, USA) to obtain gene expression ratios.