Bacterial transcriptional analysis of P. aeruginosa infection.

Invaded GFP positive (“inv”) cells were sorted 2 hours post-infection with WT or ΔalgR PAO1 GFP-expressing bacteria. RNAseq libraries were made using the scDualseq protocol and enriched for P. aeruginosa mRNA using PatH-Cap. Libraries from log phase planktonic culture grown in LB (“log”) were made using the RNAtag-Seq protocol. Raw counts, and DESeq2 analysis (log2 fold change and pajd) shown for intracellular WT vs intracellular ΔalgR bacteria and intracellular vs log phase bacteria. (XLSX)