Validated Screening Method for 81 Multiclass Veterinary Drug Residues in Food via Online-Coupling High-Throughput Planar Solid-Phase Extraction to High-Performance Liquid Chromatography–Orbitrap Tandem Mass Spectrometry

Current screening capabilities for veterinary drugs (VDs)
in foods
are limited, requiring time-consuming and expensive trace-level analyses.
For the first time, a high-throughput planar solid-phase extraction
(HTpSPE) cleanup, controlled by UV/vis/FLD imaging, was developed
for screening 81 VDs from 6 different groups (glucocorticoids, anthelmintics,
antiparasitics, coccidiostats, nonsteroidal anti-inflammatory drugs,
and antibiotics) in 4 different matrices (honey, pig muscle, cow milk,
and chicken eggs). It consumed 13 times less solvent and was more
eco-friendly and 5 times faster than routine methods. The VDs were
automatically eluted using the autoTLC–LC–MS interface,
separated online on a high-performance liquid chromatography column
via a 10-min gradient, and detected by Orbitrap high-resolution tandem
mass spectrometry. The screening method was validated according to
the latest European Commission Implementing Regulation 2021/808. Most
VDs except penicillins and cephalosporins were detected at the 5-μg/kg
level in pig muscle, cow milk, and chicken eggs and 25-μg/kg
level in honey.