Initial concentrations for molecules in the model.

Concentrations (in nM) for submembrane (S) and cytosolic (C) compartments and diffusion constants (Kdiff in µm2/s) are calculated based on molecular weight of molecular species in the HEK293 cell model (0 denotes non-diffusible molecule and NA denotes NOT APLICABLE). Initial concentrations reported in this table are extracted from output of simulations without expression of biosensor. References in Kdiff column allow comparison between experimentally measured and calculated (eq. 1) diffusion constants.