TNFα Downregulates STX17 Blocking Autophagy and Impairing Platelet Metabolism and Clot Contraction

Platelets are key players in hemostasis and thrombosis. In inflammatory conditions, platelets are hyperreactive and patients have an overal increased thrombotic risk. Our previous research demonstrated that TNFα significantly increases mitochondrial mass and platelet reactivity, emphasizing the crucial role of TNFα in modulating platelet metabolism and function.We hypothesized that TNFα disrupts platelet metabolism and function by inhibiting autophagy and mitophagy, which are critical processes for preserving mitochondrial mass, integrity, and energy production. We investigated the function, metabolism, and autophagy of human and mouse platelets in myeloproliferative neoplasms, rheumatoid arthritis, and aseptic inflammation. Our analysis reveled a blocked RNA was extracted from platelets of three TNFdARE mice, and three control littermates. Bulk RNA sequencing was performed to determine transcriptional differences in platelets due inflammatory conditions mediated by TNFα