Effect of ageing on gene expression in the African turquiose killifish retina

The mammalian central nervous system (CNS) is susceptible to age-related pathologies, resulting in progressive, irreversible disease. Neurodegenerative eye conditions, like glaucoma and age-related macular degeneneration (AMD), are on the rise due to increased life expectancy. Despite this, there are currently no long-term therapies to prevent degeneration and vision loss. The short-lived African turquoise kililfsh (Nothobranchius furzeri GRZ-AD) is an ideal genetic model for ageing studies, exhibiting rapid ageing phenotypes within its four to six-mont lifespan. Investigating the molecular consequences of ageing in the retina, we conducted bulk RNA-sequencing, revealing dysregulation of genetic pathways associated with ageing CNS and retinal diseas in the aged killifish retina. To investigate the impact of ageing on the retina of the killifish, we performed bulk RNA-sequencing on the retina of three different age groups: 6- (young), 12- (middle-aged), and 18-week-old fish (old). Per condition (age group) we sequenced 10 biological replicates. Each biological replicated consisted of the right and left retina of one female killifish.