AR in skeletal muscle. AR in skeletal muscle

Determine AR cistrome in skeletal muscle and correlate it with the presence of H3K4me2. Overall design: Muscle nuclei were extracted as described (Joshi et al., 2016). After sonication, chromatin was immunoprecipitated with anti-AR and anti-H3K4me2 antibodies. sequenced with an Illumina Hiseq 4000 as paired-end 100 bp reads, and mapped to the mm10 reference genome using Bowtie 2 (Langmead and Salzberg, 2012). Uniquely mapped reads were retained for further analysis. MACS2 algorithm (https://github.com/taoliu/MACS/) (Zhang et al., 2008) was used for peak calling with non-immunoprecipitated chromatin as control.